Molecular Biotechnology
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Molecular Biotechnology Molecular Biotechnology Principles and Applications of Recombinant DNA SIXTH EDITION An authoritative introduction to the fast-changing world of molecular biotechnology In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years.
This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances. This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as: Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2 Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.
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- Wiley Global Research (STMS)
- 9781683673668
- 9781683673644
- ePub
- 6
- Bernard R. Glick; Cheryl L. Patten
- English
- 2022-02-07
- 100
- 10
- 2
Kaflar
- Cover
- Title Page
- Copyright
- About the Companion Website
- Preface to the Sixth Edition
- 1 The Development of Molecular Biotechnology
- Emergence of Molecular Biotechnology
- Recombinant DNA Technology
- Commercialization of Molecular Biotechnology
- Concerns and Consequences
- Summary
- References
- Review Questions
- 2 Fundamental Technologies
- Molecular Cloning
- Genome Engineering Using CRISPR Technology
- Polymerase Chain Reaction
- Chemical Synthesis of Genes
- DNA Sequencing Technologies
- Sequencing Whole Genomes
- Genomics
- Summary
- References
- Review Questions
- 3 Production of Recombinant Proteins
- Protein Production in Prokaryotic Hosts
- Heterologous Protein Production in Eukaryotic Cells
- Protein Engineering
- Summary
- References
- Review Questions
- 4 Molecular Diagnostics
- Immunological Approaches To Detect Protein Biomarkers
- DNA‐Based Diagnostic Approaches
- Detecting RNA Signatures of Disease
- Biofluorescent and Bioluminescent Systems
- Summary
- References
- Review Questions
- 5 Protein Therapeutics
- Pharmaceuticals
- Enzymes
- Bacteria and Therapeutics
- Recombinant Antibodies
- Affibody Molecules
- Summary
- References
- Review Questions
- 6 Nucleic Acids as Therapeutic Agents
- Targeting Specific mRNA and DNA Sequences
- Viral Delivery Systems
- Nonviral Delivery Systems
- Gene Therapy
- Summary
- References
- Review Questions
- 7 Vaccines
- Vaccination
- Current and Future Vaccines
- Subunit and Peptide Vaccines
- Genetic Immunization: DNA Vaccines
- Engineered Attenuated Vaccines
- Vector Vaccines
- Monoclonal Antibody Passive Immunity
- Summary
- References
- Review Questions
- 8 Industrial and Environmental Uses of Recombinant Microorganisms
- Restriction Endonucleases
- Small Biological Molecules
- Microbial Degradation of Xenobiotics
- Utilization of Starch and Sugars
- Utilization of Cellulose and Hemicellulose
- Lipids from Cyanobacteria
- Hydrogen Production
- Summary
- References
- Review Questions
- 9 Large‐Scale Production of Proteins and Nucleic Acids from Recombinant Microorganisms
- Principles of Microbial Growth
- Maximizing the Efficiency of the Fermentation Process
- Bioreactors
- Typical Large‐Scale Fermentation Systems
- Harvesting Microbial Cells
- Disrupting Microbial Cells
- Downstream Processing
- Large‐Scale Production of DNA and RNA
- Summary
- References
- Review Questions
- 10 Genetic Engineering of Plants: Methodology
- Plant Transformation with the Ti Plasmid of A. tumefaciens
- Ti Plasmid‐Derived Vector Systems
- Microprojectile Bombardment
- Chloroplast Engineering
- Use of Reporter Genes in Transformed Plant Cells
- Manipulation of Gene Expression in Plants
- Production of Marker‐Free Transgenic Plants
- Summary
- References
- Review Questions
- 11 Transgenic Plants
- Insect Resistance
- Virus Resistance
- Herbicide Resistance
- Fungus and Bacterium Resistance
- Salt and Drought Stress
- Phytoremediation
- Fruits and Flowers
- Modification of Plant Nutritional Content
- Modification of Food Plant Taste and Appearance
- Plants as Bioreactors
- Edible Vaccines
- Plant Yield
- Summary
- References
- Review Questions
- 12 Transgenic Animals
- Transgenic Animal Methodologies
- Transgenic Animal Models of Human Diseases
- Animal Bioreactors for Production of Recombinant Therapeutic Proteins
- Enhancing Production Traits of Food Animals
- Gene Drives To Eradicate Vector‐Transmitted Diseases
- Summary
- References
- Review Questions
- 13 Molecular Biotechnology and Society
- Development of Guidelines for Recombinant DNA Research
- Deliberate Release of Genetically Modified Microorganisms
- Regulation of Genetically Modified Foods
- Societal Concerns about Genetically Modified Foods
- Regulation and Safety of Medical Products of Biotechnology
- Patenting Biotechnology
- Summary
- References
- Review Questions
- Amino Acids of Proteins and Their Designations
- Index
- End User License Agreement